FARMINGDALE, N.Y. -- Rickie Fowler lost the lead, the tournament and a guaranteed spot on the Ryder Cup team.Right when it looked as though Zach Johnson had played his way off the U.S. team, Fowler squandered a chance to earn the eighth and final automatic spot when he shot 39 on the back nine at The Barclays, capped off by two bogeys and a double bogey over his last four holes.Very disappointing, Fowler said. Just made a couple bad swings at the wrong time.Sunday was the final day for Americans to earn automatic spots on the team that will try to win the Ryder Cup back from Europe on Sept. 30 to Oct. 2 at Hazeltine at Minnesota.Five of the spots were already clinched going into The Barclays -- U.S. Open champion Dustin Johnson, Jordan Spieth, Phil Mickelson, PGA champion Jimmy Walker and Ryder Cup rookie Brooks Koepka.Patrick Reed moved from No. 8 to No. 4 with his one-shot victory at Bethpage Black. Brandt Snedeker and Zach Johnson kept their spots.Johnson tied for 48th, leaving himself vulnerable for being knocked out of the top eight when Fowler had a two-shot lead early in the final round. Fowler only needed to finish alone in third to overtake Johnson, and he was two shots ahead of third place with four holes to go.Thats when it all went wrong.Im just trying to win the tournament, Fowler said when asked if he knew where he stood. If I win, then it takes care of both things. Obviously, once Patrick got a little bit of a cushion, I still wanted to get the best finish I can.It will hurt, Fowler said. But its only going to make it better for next week. Ill be in a good spot.Davis Love III still has four captains picks over the next three weeks. He will make three of them after the BMW Championship on Sept. 12, and the final pick after the Tour Championship, which ends the Sunday before Ryder Cup week.Bubba Watson closed with a 66 and tied for 13th to move into ninth place, ahead of J.B. Holmes, Fowler and Matt Kuchar, who won the bronze medal at the Olympics.That gives the Americans two of this years major champions and only one rookie.Spieth, Reed, Walker and Snedeker will be playing on their second Ryder Cup team. Zach Johnson made his fourth Ryder Cup team, while Dustin Johnson is playing for the third time and Mickelson qualified on his own for the 11th time, an American record.Europes automatic nine spots were decided a week ago, and already there are five Ryder Cup rookies in Masters champion Danny Willett, Andy Sullivan, Matthew Fitzpatrick, Rafa Cabrera Bello and Chris Wood.European captain Darren Clarke makes his three picks Tuesday. 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NEW YORK -- The Latest on the U.S. Open (all times local):11:35 p.m.Serena Williams has used the cupping therapy that drew so much attention during the Olympics when Michael Phelps won gold with purple circles dotting his shoulder and back.But the 22-time major champion says its been for relaxation, not recovery.After her first-round win at the U.S. Open on Tuesday, Williams said: If I go to my lady in Palm Beach, its part of acupuncture. I love getting it; it makes me relax.She adds: I was like, `Wow, you can do that for recovery?She says she has never done it away from home before but maybe shell start. Williams says: Im always learning new things. I definitely would love to try it on the road because I love the way it feels.---11:20 p.m.Andy Murray got off to an easy start at the U.S. Open in his attempt to become the fourth man in the Open era to reach all four Grand Slam finals in a single season.The 2012 champion at Flushing Meadows and seeded No. 2 this year, Murray beat Lukas Rosol 6-3, 6-2, 6-2 on Tuesday night to get to the second round.Murray lost to No. 1 Novak Djokovic in the finals of the Australian Open in January and French Open in June, and then won his second Wimbledon title last month.Only Djokovic (last season), Roger Federer (2004, 2006 and 2007) and Rod Laver (1969, when he completed a calendar-year Grand Slam) have been to a seasons four major title matches since the professional era began in 1968.Murray has won 23 of his past 24 matches, including an unprecedented second consecutive Olympic singles gold medal at the Rio Games this month.---9:15 p.m.American Steve Johnson rallied from down two sets and a break to move on to the second round at the U.S. Open.The 19th-seeded Johnson outlasted Evgeny Donskoy 4-6, 1-6, 7-6 (2), 6-3, 6-3 in 3 hours, 13 minutes.Donskoy, ranked 79th, served for the match in the third set, when he also had six match points on Johnsons serve.Johnson, who lost in the first round at his last three U.S. Opens, routed Donskoy 6-1, 6-1 at the Olympics less than three weeks earlier. It was his second career comeback from down two sets.He had 54 unforced errors in the first three sets and 20 in the last two. Johnson next faces 2009 U.S. Open champ Juan Martin del Potro.---8:50 p.m.Serena Williams serve was in fine form in a routine first-round win over Ekaterina Makarova at the U.S. Open.The top-seeded Williams had played just three singles matches since Wimbledon because of a sore right shoulder, but it didnt show as she hit 12 aces, with only one double-fault, in a 6-3, 6-3 victory Tuesday in 63 minutes.It was a potentially tricky draw for Williams as she opened her bid for a record 23rd major title. Makarova is a two-time Grand Slam semifinalist who just missed a seed.Williams first serve averaged 108 mph, and she hit one as hard as 121 mph.---7:55 p.m.In the latest outburst by Bernard Tomic, the Australian muttered at a heckler during his U.S. Open match that he was going to perform a lewd act on that person.Tomic said after his first-round loss Tuesday that he apologized to the fan afterward. He added, though: He definitely baited me the whole set for me to say that. But I do apologize if there were people around that heard. Thats all I can say.The 17th-seeded Tomic lost 6-4, 6-3, 4-6, 7-6 (0) to 72nd-ranked Damir Dzumhur, making 78 unforced errors.The vulgar comments came during the first set as Dzumhur was trying to serve. Tomic later claimed he didnt remember what the heckler was saying to him.Tomic, 23, has attracted plenty of controversy on and off the court. He has been accused of tanking matches and suspended from Australias Davis Cup team and had multiple run-ins with police. His father, who serves as his coach, was given an eight-month suspended sentence and banned from the ATP Tour for a year in September 2013 for assaulting his sons former hitting partner.---7:10 p.m.Juan Martin del Potro, the 2009 champ, has won his opening match in his first U.S. Open since 2013.Del Potro beat fellow Argentine Diego Schwartzman 6-4, 6-4, 7-6 (3) on Tuesday after missing the tournament the last two years because of three left wrist surgeries.Del Potro is ranked just 142nd and needed a wild card to get in. But he proved with his unexpected silver medal at the Olympics that he can contend again.In his first match since losing to Andy Murray in four grueling sets in the final in Rio, del Potro showed few ill effects of his emotionally and physically draining run there.Schwartzman is ranked 73 spots better than del Potro right now, but hes never been in the top 50 or advanced past the second round at a major.---6:50 p.m.Venus Williams needed three sets to win her 18th first-round match at the U.S. Open.She defeated Kateryna Kozlova of Ukraine 6-2, 5-7, 6-4 despite 63 unforced errors.Williams led 5-2 in the third set, but the 22-year-old Kozlova pushed her to the limit in the 2-hour, 42-minute match.Williams, a two-time winner in Flushing Meadows, next faces Julia Georges, who defeated Yanina Wickmayer 6-3, 6-2.---4:50 p.m.Third-seeded Stan Wawrinka advanced to the second round of the U.S. Open with a straight-set win.TThe two-time major champion beat Fernando Verdasco 7-6 (4), 6-4, 6-4 on Tuesday despite more unforced errors than winners (37-30).dddddddddddd He saved all four break points he faced, while converting two of his five chances, which was just enough for the victory.The 32-year-old Verdasco is a former top-10 player, but he hasnt been to a major quarterfinal since 2013.---4:30 p.m.American teen Jared Donaldson has upset 12th-seeded David Goffin for his first career win over a top-30 player.The 19-year-old qualifier won 4-6, 7-5, 6-4, 6-0 in the first round of the U.S. Open on Tuesday for his first victory at a major. He had lost in straight sets in his two previous Grand Slam matches, both at Flushing Meadows. Donaldson is ranked a career-best 122nd.Goffin reached his first major quarterfinal at this years French Open.---4:10 p.m.American Sam Querrey couldnt build on his upset of Novak Djokovic in his next Grand Slam.The 29th-seeded Querrey lost to Janko Tipsarevic 7-6 (4), 6-7 (0), 6-3, 6-3 in the first round of the U.S. Open on Tuesday. It was a tough opening matchup for Querrey -- Tipsarevic is a former top-10 player whose ranking is down to 250th after a series of injuries. A two-time quarterfinalist at Flushing Meadows, Tipsarevic got in on a protected ranking.Hed lost five straight Grand Slam matches dating to the 2013 U.S. Open before Tuesday.Querrey stunned the top-ranked Djokovic in the third round at Wimbledon en route to his first major quarterfinal. But in six of his past eight Grand Slams, hes lost to a lower-ranked opponent in the first round.Hes also dropped seven of his past eight meetings with Serbian opponents -- the only win that upset of Djokovic at Wimbledon.---3:30 p.m.Samantha Stosur has advanced to the second round of the U.S. Open with a 7-5, 6-7 (4), 6-1 win over Camila Giorgi.The 2011 U.S. Open champion needed three sets against Giorgi, who had an ace to win the second-set tiebreaker. But the 67th-ranked Giorgi got off to a slow start in the third, with Stosur taking a 4-0 lead in the deciding set.Giorgi capitalized on only 3 of 14 break-point opportunities. She won 15 of 20 net points, but finished with 45 unforced errors.Stosur will next face Zhang Shuai, who defeated Ellen Perez 6-1, 6-1.----1:50 p.m.Rio Olympics bronze medalist and 2014 U.S. Open runner-up Kei Nishikori has avoided what would have been his fourth first-round exit in his past six appearances at Flushing Meadows.Nishikori, who earned Japans first Olympic tennis medal since 1920 a little more than two weeks ago, advanced in New York on Tuesday by beating Benjamin Becker 6-1, 6-1, 3-6, 6-3.The sixth-seeded Nishikori reached his first Grand Slam final at the U.S. Open two years ago, losing to Marin Cilic at that stage. But he exited in the first round in 2011, 2013 and 2015.Becker, a German who won on NCAA singles title at Baylor University, has lost his past 15 matches against opponents ranked in the top 10.---1:30 p.m.Ana Ivanovic is out of the U.S. Open in the first round for the second straight year after a 7-6 (4), 6-1 loss to Denisa Allertova of the Czech Republic.Ivanovic is a former No. 1 player and 2008 French Open winner who has slipped to No. 31 in the rankings.The Serb was serving for the first set at 6-5 but struggled with her serve and faltered in the tiebreaker. She finished with seven double-faults and 41 unforced errors.At the Open, she has reached the fourth round or better five times, including a career-best quarterfinal run in 2012.The 89th-ranked Allertova improved to 7-1 in first-round matches in majors, making her second main-draw appearance at the Open.----12:30 p.m.Simona Halep gave herself less than 9 for her quick work against Kirsten Flipkens, 6-0, 6-2, in the first match on Arthur Ashe Stadium.The Romanian was ahead 6-0, 5-0 with countrywoman Nadia Comaneci cheering her on. Comaneci was the first gymnast to score a perfect 10 at the 1976 Montreal Olympics.Halep says she wasnt perfect, but pleased with how she played against the Belgian. She got 69 percent of her first serves in and won 12 of 15 points at the net.Halep, who reached the semifinals of the U.S. Open last year, will next face Lucie Safarova, a 6-4, 6-4 winner over Daria Gavrilova.----12:05 p.m.Venus and Serena Williams are among the featured matches on Arthur Ashe Stadium at the U.S. Open.Venus will take on Kateryna Kozlova of Ukraine in the afternoon Tuesday before her top-ranked sister plays Ekaterina Makarova of Russia in the night match at the stadium with the new retractable roof.Serena has been bothered by a shoulder injury, playing only three matches since winning Wimbledon for her 22nd Grand Slam title. She faces a tough opening opponent in Makarova, who beat her at the 2012 Australian Open.Kirsten Flipkens of Belgium and Simona Halep of Romania have started play on Ashe, followed by Fernando Verdasco of Spain against Stan Wawrinka of Switzerland.Andy Murray of Britain will face Lukas Rosol of the Czech Republic in the other evening match. ' ' '